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recombinant human podoplanin rfc  (TargetMol)


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    Structured Review

    TargetMol recombinant human podoplanin rfc
    H igh-throughput screening based on CLEC-2 and <t>podoplanin</t> interaction. ( A ) Schematic representation of ALPHA screen based on podoplanin and CLEC-2 interaction. ALPHA screen assay is based on the proximity of the donor and acceptor beads mediated by the protein–protein interaction. After laser excitation, podoplanin-conjugated donor beads transform ambient oxygen to singlet oxygen, which is absorbed by CLEC-2-conjugated nickel acceptor beads, then ALPHA signal is emitted and detected by the plate reader. ( B-i ) Scatter plot showing the effect of each compound on the ALPHA signal values emitted by CLEC-2 and podoplanin interaction; dots below the cut off ( red line ) represent compounds that significantly decreased signal and potential hits. ( B-ii ) Scatter plot showing TruHits assay to identify potential false-positive compounds on the screening; dots over the cut-off indicate potential hits. ( C ) The dose–response curves of potential hits and IC50 of each potential compound (i–vi). C-i and C-ii show the dose–response curves of the two most potent compounds, further evaluated them in functional assays. shows a chemical description of the compounds tested.
    Recombinant Human Podoplanin Rfc, supplied by TargetMol, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+podoplanin+rfc/Platelet+Factor+4+(58-70)%2C+human/pmc09393086-21-10-4
    Average 92 stars, based on 3 article reviews
    recombinant human podoplanin rfc - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "Katacine Is a New Ligand of CLEC-2 that Acts as a Platelet Agonist"

    Article Title: Katacine Is a New Ligand of CLEC-2 that Acts as a Platelet Agonist

    Journal: Thrombosis and Haemostasis

    doi: 10.1055/a-1772-1069

    H igh-throughput screening based on CLEC-2 and podoplanin interaction. ( A ) Schematic representation of ALPHA screen based on podoplanin and CLEC-2 interaction. ALPHA screen assay is based on the proximity of the donor and acceptor beads mediated by the protein–protein interaction. After laser excitation, podoplanin-conjugated donor beads transform ambient oxygen to singlet oxygen, which is absorbed by CLEC-2-conjugated nickel acceptor beads, then ALPHA signal is emitted and detected by the plate reader. ( B-i ) Scatter plot showing the effect of each compound on the ALPHA signal values emitted by CLEC-2 and podoplanin interaction; dots below the cut off ( red line ) represent compounds that significantly decreased signal and potential hits. ( B-ii ) Scatter plot showing TruHits assay to identify potential false-positive compounds on the screening; dots over the cut-off indicate potential hits. ( C ) The dose–response curves of potential hits and IC50 of each potential compound (i–vi). C-i and C-ii show the dose–response curves of the two most potent compounds, further evaluated them in functional assays. shows a chemical description of the compounds tested.
    Figure Legend Snippet: H igh-throughput screening based on CLEC-2 and podoplanin interaction. ( A ) Schematic representation of ALPHA screen based on podoplanin and CLEC-2 interaction. ALPHA screen assay is based on the proximity of the donor and acceptor beads mediated by the protein–protein interaction. After laser excitation, podoplanin-conjugated donor beads transform ambient oxygen to singlet oxygen, which is absorbed by CLEC-2-conjugated nickel acceptor beads, then ALPHA signal is emitted and detected by the plate reader. ( B-i ) Scatter plot showing the effect of each compound on the ALPHA signal values emitted by CLEC-2 and podoplanin interaction; dots below the cut off ( red line ) represent compounds that significantly decreased signal and potential hits. ( B-ii ) Scatter plot showing TruHits assay to identify potential false-positive compounds on the screening; dots over the cut-off indicate potential hits. ( C ) The dose–response curves of potential hits and IC50 of each potential compound (i–vi). C-i and C-ii show the dose–response curves of the two most potent compounds, further evaluated them in functional assays. shows a chemical description of the compounds tested.

    Techniques Used: Functional Assay

    Katacine binds to the same binding site as rhodocytin based on molecular binding site prediction. Molecular docking has been performed using AutoDock vina and ADT for protein preparation. Grid boxes for binding prediction have covered the extracellular domain of CLEC-2, using the crystal structure of human CLEC-2 (PDB: 2C6U). Katacine can bind to a positive charge surface rich of arginine in the CLEC-2 extracellular domain (Arg-107, Arg-118, Arg-152, Arg-157) previously reported as the binding site for podoplanin and rhodocytin (enclosed in a red circle ), with a predicted binding energy of −6.6 kcal/mol. ADT, AutoDock tools.
    Figure Legend Snippet: Katacine binds to the same binding site as rhodocytin based on molecular binding site prediction. Molecular docking has been performed using AutoDock vina and ADT for protein preparation. Grid boxes for binding prediction have covered the extracellular domain of CLEC-2, using the crystal structure of human CLEC-2 (PDB: 2C6U). Katacine can bind to a positive charge surface rich of arginine in the CLEC-2 extracellular domain (Arg-107, Arg-118, Arg-152, Arg-157) previously reported as the binding site for podoplanin and rhodocytin (enclosed in a red circle ), with a predicted binding energy of −6.6 kcal/mol. ADT, AutoDock tools.

    Techniques Used: Binding Assay

    Related Articles

    Recombinant:

    Article Title: Katacine Is a New Ligand of CLEC-2 that Acts as a Platelet Agonist
    Article Snippet: .. Katacine was purchased from TargetMol (Boston, Massachusetts, United States), and recombinant human podoplanin-rFc and recombinant human CLEC-2–6xHis (Ser55-Pro229) were expressed in house HEK293T cells. .. Recombinant human CLEC-2 was also purchased from R&D Systems (Minnesota, United States) for optimization of the screening.



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    recombinant human podoplanin rfc  (TargetMol)
    92
    TargetMol recombinant human podoplanin rfc
    H igh-throughput screening based on CLEC-2 and <t>podoplanin</t> interaction. ( A ) Schematic representation of ALPHA screen based on podoplanin and CLEC-2 interaction. ALPHA screen assay is based on the proximity of the donor and acceptor beads mediated by the protein–protein interaction. After laser excitation, podoplanin-conjugated donor beads transform ambient oxygen to singlet oxygen, which is absorbed by CLEC-2-conjugated nickel acceptor beads, then ALPHA signal is emitted and detected by the plate reader. ( B-i ) Scatter plot showing the effect of each compound on the ALPHA signal values emitted by CLEC-2 and podoplanin interaction; dots below the cut off ( red line ) represent compounds that significantly decreased signal and potential hits. ( B-ii ) Scatter plot showing TruHits assay to identify potential false-positive compounds on the screening; dots over the cut-off indicate potential hits. ( C ) The dose–response curves of potential hits and IC50 of each potential compound (i–vi). C-i and C-ii show the dose–response curves of the two most potent compounds, further evaluated them in functional assays. shows a chemical description of the compounds tested.
    Recombinant Human Podoplanin Rfc, supplied by TargetMol, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+podoplanin+rfc/Platelet+Factor+4+(58-70)%2C+human/pmc09393086-21-10-4
    Average 92 stars, based on 1 article reviews
    recombinant human podoplanin rfc - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    H igh-throughput screening based on CLEC-2 and podoplanin interaction. ( A ) Schematic representation of ALPHA screen based on podoplanin and CLEC-2 interaction. ALPHA screen assay is based on the proximity of the donor and acceptor beads mediated by the protein–protein interaction. After laser excitation, podoplanin-conjugated donor beads transform ambient oxygen to singlet oxygen, which is absorbed by CLEC-2-conjugated nickel acceptor beads, then ALPHA signal is emitted and detected by the plate reader. ( B-i ) Scatter plot showing the effect of each compound on the ALPHA signal values emitted by CLEC-2 and podoplanin interaction; dots below the cut off ( red line ) represent compounds that significantly decreased signal and potential hits. ( B-ii ) Scatter plot showing TruHits assay to identify potential false-positive compounds on the screening; dots over the cut-off indicate potential hits. ( C ) The dose–response curves of potential hits and IC50 of each potential compound (i–vi). C-i and C-ii show the dose–response curves of the two most potent compounds, further evaluated them in functional assays. shows a chemical description of the compounds tested.

    Journal: Thrombosis and Haemostasis

    Article Title: Katacine Is a New Ligand of CLEC-2 that Acts as a Platelet Agonist

    doi: 10.1055/a-1772-1069

    Figure Lengend Snippet: H igh-throughput screening based on CLEC-2 and podoplanin interaction. ( A ) Schematic representation of ALPHA screen based on podoplanin and CLEC-2 interaction. ALPHA screen assay is based on the proximity of the donor and acceptor beads mediated by the protein–protein interaction. After laser excitation, podoplanin-conjugated donor beads transform ambient oxygen to singlet oxygen, which is absorbed by CLEC-2-conjugated nickel acceptor beads, then ALPHA signal is emitted and detected by the plate reader. ( B-i ) Scatter plot showing the effect of each compound on the ALPHA signal values emitted by CLEC-2 and podoplanin interaction; dots below the cut off ( red line ) represent compounds that significantly decreased signal and potential hits. ( B-ii ) Scatter plot showing TruHits assay to identify potential false-positive compounds on the screening; dots over the cut-off indicate potential hits. ( C ) The dose–response curves of potential hits and IC50 of each potential compound (i–vi). C-i and C-ii show the dose–response curves of the two most potent compounds, further evaluated them in functional assays. shows a chemical description of the compounds tested.

    Article Snippet: Katacine was purchased from TargetMol (Boston, Massachusetts, United States), and recombinant human podoplanin-rFc and recombinant human CLEC-2–6xHis (Ser55-Pro229) were expressed in house HEK293T cells.

    Techniques: Functional Assay

    Katacine binds to the same binding site as rhodocytin based on molecular binding site prediction. Molecular docking has been performed using AutoDock vina and ADT for protein preparation. Grid boxes for binding prediction have covered the extracellular domain of CLEC-2, using the crystal structure of human CLEC-2 (PDB: 2C6U). Katacine can bind to a positive charge surface rich of arginine in the CLEC-2 extracellular domain (Arg-107, Arg-118, Arg-152, Arg-157) previously reported as the binding site for podoplanin and rhodocytin (enclosed in a red circle ), with a predicted binding energy of −6.6 kcal/mol. ADT, AutoDock tools.

    Journal: Thrombosis and Haemostasis

    Article Title: Katacine Is a New Ligand of CLEC-2 that Acts as a Platelet Agonist

    doi: 10.1055/a-1772-1069

    Figure Lengend Snippet: Katacine binds to the same binding site as rhodocytin based on molecular binding site prediction. Molecular docking has been performed using AutoDock vina and ADT for protein preparation. Grid boxes for binding prediction have covered the extracellular domain of CLEC-2, using the crystal structure of human CLEC-2 (PDB: 2C6U). Katacine can bind to a positive charge surface rich of arginine in the CLEC-2 extracellular domain (Arg-107, Arg-118, Arg-152, Arg-157) previously reported as the binding site for podoplanin and rhodocytin (enclosed in a red circle ), with a predicted binding energy of −6.6 kcal/mol. ADT, AutoDock tools.

    Article Snippet: Katacine was purchased from TargetMol (Boston, Massachusetts, United States), and recombinant human podoplanin-rFc and recombinant human CLEC-2–6xHis (Ser55-Pro229) were expressed in house HEK293T cells.

    Techniques: Binding Assay